Abstract
This is comparative study of developing bioethanol from age old waste rice straw (WRS) collected from thatched roofs pretreated with tartaric acid and sodium chlorite separately. WRS of different ages, collected from nearby village, they were dried, grinded and screened. Proximate analysis was conducted by (Neutral detergent fibre) NDF and (Acid detergent fibre) ADF methods for estimating the hemicellulose, cellulose and lignin content of variable age (not more than 6, 12, 24, 36 and 48 months), with least content 11.35% in 48 months age old sample. Pre-treatment was conducted on least lignin content biomass sample by using different concentrations of tartaric acid and sodium chlorite under variable physical parameters such as soaking temperature, soaking time and agitation speed which were optimized by response surface methodology design tool, followed by moist heat treatment. The sample resulting best lignin degradation estimated by total phenol content was subjected to saccharification by using both xylanase and cellulase. The time yielding maximum hexose and pentose was 36th hour resulting in 494.8 mg/g and 274.9 mg/g glucose and 134.8 mg/g and 124 mg/g xylose for tartaric acid and sodium chlorite pretreated samples respectively. After saccharification the hydrolysate was collected and set for fermentation by Pichia stipitis. Ethanol yield was obtained to be 20.226% and 25.467%.
Paper version not known (
Free)
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call