Comparative study of the cytoto xicity and apoptotic effect of beet root, and silica-beet nanopartic les with 5-FU nanopartic les against Scc-090 cell line

Abstract

Objective: Nanoparticle delivery systems can be used to target anticancer drugs to tumortissues. Beet root appears to be a powerful dietary source of antioxidant agent with anti-cancereffect due to ability to scavenge free radicals. Application of silica-nanoparticles (SiNP) in cancertreatment are promising, with increased data suggesting anti-proliferative effects in cancer cells.The aim of our study was to compare the cytotoxic effect of the beet root NP, silica-beet NP with5fu (flourarcil) NP , a chemotherapeutic agent against tongue carcinoma cell line scc-090.Methods: Cell Line cells were obtained from American Type Culture Collection , cells werecultured using DMEM (Invitrogen/Life Technologies). Beet root nanoparticles Si-beet NP and 5FUdrug NP were used. The effect of the tested materials were investigated by MTTassay and DNAfragmentation (DPA method).Results: The MTT cytotoxicity assay revealed that beat root (nano) and Si-beat roots (nano)have obbvious cytotoxicity on SS C-090 cancer cell lines at low significant IC50 value as 0.73μg/ml and 2.94 μg/ml respectively. While 5FU drug revealed obvious cytotoxicity on SSC-090 cancercell lines at higher value IC50 8.83 ug/ml. these results denote that low doses of beat root (nano)may exert remarkable cell death on tongue cancer cells line scc-090.Conclusion: Our findings demonstrate that beet root NP could be capable of inducing cancercell death with prominent apoptosis at very low ic50 value 0.73μg/ml against oral tongue carcinomaSCC-090 cell lines.