Comparative Study of Retention of Glucosinolates and Inorganic Ions on Selected Silica‐Based and Polymer‐Based Ion Exchange Columns

Abstract

The retention of two glucosinolates [Sinigrin (SIN), Glucotropaeolin (GTL)] and oxalate as organic anions and inorganic anions (chloride, nitrate, sulfate) were studied on three anion exchangers, e.g., Vydac 302 IC (250 × 4.6 mm I.D.), Dionex Ion Pac (250 × 4 mm I.D.), and Waters IC Pac HR (75 × 4.6 mm I.D.) under isocratic elution conditions. The two glucosinolates carry the same functional group (‒OSO3 −), however, do not have the same retention in anion exchange chromatography. The plots of capacity factors k of organic and inorganic anions vs. the reciprocal of eluent ion concentration show good linearity. From the slope and y‐intercept data, the major retention mechanisms are interpreted as ion exchange and some other interactions. The influence of organic modifier addition to the aqueous buffered mobile phase is also investigated. An Evaporative Light Scattering Detector (ELSD) was used.