Abstract
Promoter sequences of three anther-specific genes, each of which shows sequence identity to lipid transfer protein (LTP12), xyloglucan endotransglucosylase/hydrolase (XTH3), and polygalacturonase (PGA4), were obtained from Arabidopsis thaliana, fused to the β-glucuronidase (GUS) gene, and then introduced into A. thaliana. Histochemical GUS assay showed that the PGA4 promoter was active in the tapetum at the bicellular pollen stage and in tricellular pollen. The promoter of LTP12 and XTH3 directed GUS expression exclusively in the tapetum. The LTP12 promoter was activated from the uninucleate microspore stage, while the XTH3 promoter was activated from the bicellular pollen stage. This type of activation pattern at the late developmental stage of the tapetum has not been reported previously. The promoter sequences employed in this study will be useful for the characterization of genes differentially expressed in anthers.
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