Comparative Study of Nutritional Values of Edible Viscera Mediterranean Mollusks Gastropods hexaplex trunculus and bolinus brandaris. Hypobranchial glands inhibit Human Glioblastoma U87 Tumor Cells Adhesion and Proliferation

Abstract

This study aims first to identify nutritionals values of edible mix Muscle Hypobranchial Gland MHG, fresh and cooked extract from internal organs of two gastropods mollusks Hexaplex trunculus HT and Bolinus brandaris BB males and females. Second, to characterize and evaluate the role of hypobranchial glands extract HBGE of these mollusks gastropods on adhesion and cell proliferation of human glioblastoma tumor cells U87. Biochemical analyzes show that fresh MHG, HT and BB are rich in Water 82.52% in HT males, 79.23% in HT females. They are rich in Proteins 12.79 % in BB females to 10.30% in HT females) and in Energy value 79.14 kcal in BB females to 69.48kcal in HT males. They contain fewer carbohydrates 6.27% in BB males to 5.40% in HT males, less total ash 1.52% in HT females to 1.11% in HT males and less lipids 2.55% in HT females to 0.74% in BB males. Further, HBGE of HT inhibited U87 cell adhesion to fibrinogen at 15 mg/ml. This inhibition was accelerated at 20 mg/ml without exceeding 50% of inhibition. HBGE of BB reduced glioblastoma cells adhesion upon to 5 mg/ml with IC50 values of 9 mg/ml for male and 20 mg/ml for female. U87 cells treated with HBGE HT and BB decreased significantly cell proliferation by 50% and 70% respectively. Our results lead us to encourage the use of these marine snails, source of protein and high energy values. Moreover, HBGE may have the potential to serve as a template for future anticancer-drug development. Keywords : Mix Muscle- Hypobranchial Gland, Hexaplex Trunculus, Bolinus Brandaris, Nutritional Values, Glioblastoma U87 Cells, Cell Adhesion and Proliferation.