Ethanol extraction of orange peels from agricultural waste and its activity against four species of the bacteria

Abstract

The present study was amid the Hot extraction of orange peels. It evaluated its ability to inhibit the bacteria growth against four types, i.e. Bacillus anthracis (B anthracis), Streptococcus pyogenes (S. pyogenes), Yersinia pestis (Y. pestis) and Klebsiella pneumonia (K. pneumonia). Hot-extraction method was used to extract orange peels (EPO) by using ethanol as a solvent. Five concentrations of the extract orange peels were treated against the bacteria. The results showed significant effects against all types of bacteria, where the results were 15mm against B. anthracis, while S. pyogenes showed more effect, reaching 17mm. Moreover, the measurements of the EPO zone against Y. pestis were 12mm, while its zone against K. pneumonia was 15mm. The obtained results can give clear evidence of the ability of EPO to be used as an antibacterial agent. The current study has achieved positive results by utilizing agricultural waste and converting it into useful materials in the biological field, as well as reducing environmental pollution.