Abstract
The importance of Mesenchymal stem cells (MSCs) represents a favorable tool for new clinical concepts in supporting tissue engineering and cellular therapy. Bone marrow (BM) was considered important source contain mesenchymal stem cells .Another promising source of MSCs is adipose tissue (AT). MSCs derived from these sources compared regarding morphology, the success rate of isolating MSCs, expansion potential by rate of colony forming and immune phenotype. The obtained results from this study showed no obvious considerable differences concerning the morphology and immune phenotype of the MSCs derived from these sources were obvious. Differences observed concerning to the success rate of isolating MSCs, which was approximately more than 90% for BM, while it reached about 70% for AT after seven days of culturing, as well as the rate of colony forming was lower in AT cells in comparison to that obtained in BM at the same period. However, AT-MSCs could be required longest time to complete monolayer confluence, whereas BM-MSCs had the shortest proliferation period. Cells from both sources determined according to immunohistochemistry by CD105+ and CD34.¯ Conclusions revealed that MSCs can easily and successfully obtained from bone marrow and adipose tissues, and both tissues appears suitable sources of stem cells for potential use in regenerative medicine, repairing damaged tissue nevertheless the BM-MSCs more effectual in expansion and proliferation.
Highlights
Stem cell therapies can provide an alternative approach for repair and regeneration of tissues and organs
Collection, Isolation and separation of bone marrow mesenchymal stem cells (BM-Mesenchymal stem cells (MSCs)) Bone marrow cells were isolated from the femur of 4-8 week-old, mice male at weights ranging from 10-15g
Isolated whole bone marrow cells were resuspended in growth culture medium (Minimal Essential Medium) MEM supplemented with 15 % FBS(Fetal bovine serum), 1 % Ampicllin and Streptomycin, the collected BM cells were centrifuged at 1000 rpm for 10 min at 18°C, supernatant was aspirated and the pellet was washed twice with PBS according to Fortier,et al [10]
Summary
Stem cell therapies can provide an alternative approach for repair and regeneration of tissues and organs. In addition to adult mesenchymal stem such as bone marrow mesenchymal stem cells (BMMSCs), adipose-derived stem cells (ADSCs) have attracted increasing attention because they are less to obtain, can be safely transplanted into an autologous or allogeneic host and exhibit higher cell activity [4,5,6]. According to the International Society for Cellular Therapy, MSCs are defined as being (i) plasticadherent in the standard cell culture condition, (ii) multipotent, i.e., able to differentiate into osteoblasts, adipocytes and chondrocytes in vitro and (iii) positive for CD90 and CD105, and negative for CD34, CD45 in their cell surface immunophenotype [7]. The aim of this study was to compare the efficiency isolation of MSCs from the two sources, at identical conditions(growth medium, cells culturing and replacement medium) in vitro with respect to their morphology, rate of isolating of cells, expansion of colony forming characteristics and immunophenotype
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