Comparative study of different protocols for isolation of RNA from polysaccharides and polyphenolics rich plant tissues

Abstract

The isolation of high quality ribonucleic acid (RNA) from plant has been a difficult problem due to presence of high amount of polysaccharides, polyplenolics and nucleases that mediate RNA degradation. After comparative analysis of four popular protocols for RNA isolation, a modified method was developed which gave maximum yield with purity. Modified method is not only suitable for the isolation of intact RNA from immature seeds of legumes, containing high amount of starch, polyphenolics and several nucleases but also works successfully with other plant organs like leaves, epicotyls and callus. The yield of total RNA extracted by this method varies from 71 to 417mg/g tissues. Key words: RNA isolation, polysaccharides and polyphenolics rich plant tissues, pegionpea and immature seeds.