Comparative Study involved in Antioxidant and Antimicrobial assessment of six brut extracts from Prunus persica L. leaves

Abstract

The objectives of this study were to evaluate the biological properties of principal secondary metabolites of Prunus persica L. leaves using different in vitro assays. The chloroformic extract contained the highest levels of total phenolic, flavonoids, tannins and flavonolscontents with 116.83±4.31mg GAE/g DW, 637.78±46.01mg QRE/g DW, 141.89±5.63mg CAE/g DW and 91.32±4.36mg QRE/g DW respectively. The IC50 values based on the DPPH 1.03±0.10mg/mL and ABTS 0.21±0.06mg/mL were high in aqueous extract. The same extract demonstrated best antioxidant activity inhydrogen peroxide assay with 31.53±0.37% better than BHT, andin FRAP assay with an amount 253.33±128.32mg Fe(II)E/g. while the chloroformic and methanolic extracts showed an effective antioxidant activity on TAC and reducing power. A strong correlation was found between phenolic contents and IC50 values obtained by different antioxidant assays. The TEC50 reaches equilibrium at 7.70 and 8 min in chloroformic extract and ascorbic acid respectively in kinetic behaviour. Furthermore, the ethanolic extract showed marked antimicrobial properties against C. albican ATCC 10231 and against L. monocytogenes where the MIC and MBC were determined at 12.5mg/mL. In addition, the fungal index reached 20.59±17.14% against P. glandicola.