Evaluation of antioxidant, anti-inflammatory and adipocyte differentiation inhibitory potential of Ziziphus mauritiana bark extract

Abstract

IntroductionZiziphus mauritiana is a shrubs belonging to family Rhamnaceae and distributed in warm temperature zone from western Africa to India. Earlier, anti-cancer, anti-inflammatory and anti-diabetic activities were attributed to Z. mauritiana. Materials and methodsThe antioxidant activity of chloroform, ethanol and aqueous extracts of Z. mauritiana bark was assessed by ABTS + decolourization assay. The anti-inflammatory activity was studied in Wistar rats by Carrageenan induced paw edema assay. The adipocyte differentiation (in 3T3-L1 adipocyte cell) and glucose uptake (in CHO-HIRC-mycGLUT4e GFP cells) assay of Z. mauritiana bark aqueous extract was performed. ResultsThe chloroform, ethanol and aqueous extracts showed good antioxidant activity. The anti-inflammatory activity (27.83% reduction in inflammation) was observed with chloroform extract at the concentration of 200 mg/kg body weight when administered orally in rats. The aqueous extract showed reduction in number and size of the oil droplets in 3T3-L1 adipocyte cell cytoplasm which was found to be dose dependent. The aqueous extract stimulated the glucose uptake in the CHO-HIRC-mycGLUT4e GFP cells which was comparable to insulin. ConclusionThe chloroform, ethanol and aqueous extracts showed good antioxidant activity. The anti-inflammatory potential was shown by chloroform extract only. The adipocyte differentiation inhibition and glucose uptake in CHO-HIRC-mycGLUT4e GFP cells was induced by aqueous extract.