Abstract
A manipulation of four circles arranged along the Cartesian coordinates in uniform circular aperture is compared with selected four blood cells forming the object using the technique of speckle photography. The aperture model is considered have two- fold symmetry. A diffuser is placed in the same plane of the object or the model of four circles in order to get the modulated speckle pattern which is the Fourier spectrum of object information convoluted with the Fourier spectrum of the random diffuser. The modulated speckle pattern is smeared by the Point Spread Function computed from the diffraction corresponding to the whole circular aperture. The reconstruction process gave nearly similar speckle images little degraded by the noise originated from the diffuser. The Point Spread Function corresponding to the model of four circles is computed analytically and also using the FFT technique. A comparison is made with the Fourier spectrum of the blood cells and its corresponding autocorrelation function giving nearly similar results. We can acquire the erythrocytes blood cells in the imaging plane by operating the FFT upon the modulated speckle images. The reconstructed images of blood cells are affected by the noise originated from the diffuser. A Mat- Lab code is made in the construction of the aperture and all the images computed in the processing.
Highlights
A lot of work made by many authors applied on medical images concerning the speckle images used only circular window
In the past decade [8, 9] suggested the linear, the quadratic, and the B/W apertures of constant annular width in the study of the Confocal Laser Scanning Microscope. They computed the Point Spread Function (PSF) and the image of a point in case of conventional and confocal microscopes and obtained resolution improvement compared with circular aperture manipulations
We investigate the speckle images for the microscopic blood cells using apertures of two- fold symmetry composed of four circles instead of clear circular window
Summary
A lot of work made by many authors applied on medical images concerning the speckle images used only circular window. In the past decade [8, 9] suggested the linear, the quadratic, and the B/W apertures of constant annular width in the study of the Confocal Laser Scanning Microscope. They computed the Point Spread Function (PSF) and the image of a point in case of conventional and confocal microscopes and obtained resolution improvement compared with circular aperture manipulations. We investigate the speckle images for the microscopic blood cells using apertures of two- fold symmetry composed of four circles instead of clear circular window.
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