Abstract
Objective To compare the difference between electrochemiluminescence immunoassay (ECLIA) and radioactive receptor assay(RRA)in measurement of serum thyrotropin receptor antibody(TRAb),and to explore the clinical value and feasibility of ECLIA in TRAb.Methods Seventy-five patients were selected with symptomatic Graves'disease (GD)group 32 cases,GD in remission group 23 cases and control group 20 cases.Both ECLIA and RRA were used for the measurement of serum TRAb in all the patients.Results ①The values of TRAb with ECLIA and RRA in group of symptomatic GD were higher than those in the other two groups ( F=1 1.814,F=3.404,P＜0.05).② The positive and negative coincidence rate between the two methods were 95.7％ and 73％,respectively.There was no significant difference of the positive rate between the two methods in both groups of symptomatic GD and control (x2=3.691,x2=1.026,P＞0.05),but great difference in group of GD in remission,in which it was higher with ECLIA than with RRA (x2=5.440,P＜0.05).③ There was a good correlation between the two methods( r=0.705,P＜0.01),while the value detected by ECLIA was lower than that by RRA (Z=-4.399,P＜0.01).Conclusion ECLIA has the advantages of simple,convenient,timesaving,high sensitivity,and complete automatization,which is more suitable for clinical monitoring of serum TRAb for the patients with GD. Key words: Graves disease ; Antibody, thyrotropin receptor ; Chemiluminescent measurements; Radioligand assay
Published Version
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