Abstract
Objective: Our main aim is to evaluate the comparative in vitro antioxidant and DNA damage protection activity study with different extracts of tubers of Arisaema tortuosum.
 Methods: A. tortuosum (Wall.) Schott. (Araceae family) is commonly known as whipcord cobra lily, used in India as traditional medicine. In vitro, antioxidant activity was done by six methods including determination of total phenolic and total flavonoid compounds. The DNA damage was estimated by standard diphenylamine reaction.
 Results: Methanolic extract of tubers of A. tortuosum has been found rich in phenolic and flavonoid compounds when compared with other extracts. Methanolic extract of rhizomes of A. tortuosum showed potent antioxidant activity at a concentration of 400 μg/mL and potent the DNA damage protecting activity at a concentration of 50 μg/mL.
 Conclusion: Comparative study against oxidative damage and protection to the DNA damage protection activity of the different extracts of tubers of A. tortuosum (Wall.) Schott. showed that methanolic extract is most potent, though ethyl acetate extract also showed activity.
Highlights
Oxidative stress is the imbalance into the free radicals production and the body’s ability to use the antioxidants to counteract the harmful effects [1]
Our results demonstrated that the methanolic extracts of leaf and root of A. tortuosum possess free radical scavenging activity in vitro models such as DPPH, ABTS+, reducing power activity, and hydroxyl radical scavenging activity
Total phenolic content (TPC) and total flavonoid content (TFC) Among different extracts, methanolic extract (MEAT), the TPC was found from the calibration curve Y = 0.700X–0.869 [R2 = 0.921] as 120.26 mg GAE/g in terms of gallic acid equivalent and the TFC was found from the calibration curve Y = 0.117X−0.077 [R2 = 0.967], content was found to be 167.27 mg RUE/g in terms of rutin equivalent (Table 1)
Summary
Oxidative stress is the imbalance into the free radicals production and the body’s ability to use the antioxidants to counteract the harmful effects [1]. Interaction between the free radicals which is produced due to oxidation with the molecules within our cells results in damage to nearby cells, mitochondria, and DNA. Antioxidants in food play a key role in combat with oxidative stress. Antioxidant compounds such as polyphenols, phenolics, and flavonoids are very often found in plants that are reported to have different pharmacological effects as well as antioxidant activity [2]. Shreds of evidence showed the extremely large promising of traditional medicinal plants for their pharmacological activities and antioxidant principles [3]
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