Comparative studies on the catalatic reaction between red cell suspension and hemolysate.

Abstract

1. The rate constant of the catalatic reaction of hemolysate is only a few times as large as that of the catalatic reaction of red cell suspension.2. The activity of hemolysate catalase once treated with H2O2 is reduced in proportion to the concentrations of H2O2 previously added. On the contrary, the velocity of the catalatic reaction of red cell suspension once treated with H2O2 is a few times as large as that in the original red cell suspension.3. When the amount of red cell is small in both red cell suspension and hemolysate, a retarded stage appears in their catalatic reactions, but in case of red cell suspension, the acceleration of hydrogen peroxide decomposition appears at the same time.4. The rate of catalatic reaction in red cell suspension seems to be determined by the rate of diffusion, which may be increased by H2O2.