Comparative Studies on Physical and Chemical Property of Avain Eggs

Abstract

Horizontal polyacrylamide gradient gel electrophoretograms of egg yolk proteins of Galliformes bird species: chicken, quail, golden pheasant and silver pheasant, Anseriformes bird species: duck and muscovy duck, and a Columbiformes bird species: pigeon were studied. A step gradient gel of 8, 4 and 10% acrylamide concentration was used. The egg yolk protein bands were separated into 10 electrophoretic zones (Nos. 1-10) in 10% gel.Different electrophoretic patterns were observed in the different bird species. In Galliformes and Anseriformes, zone No. 5 was the most dense bands and No. 6 was the second most dense bands. The electrophoretograms of the pigeon was much different from those of Galliformes and Anseriformes bird species.The zone Nos. 1, 2, 3 and 8 of chicken egg yolk, the protein polymorphisms were observed. Proteins of zone No. 8 were transferrins with 3 valiants which were controlled by 2 autosomal codominant alleles: TfB and TfC. The gene frequency of TfB and TfC was 0.844 and 0.156, respectively. Zone No. 3 had 3 valiants controlled by 2 autosomal codominant alleles: Pa-1A and Pa-1B. The gene frequency of Pa-1A and Pa-1B was 0.244 and 0.756, respectively. The protein polymorphisms in zone Nos. 1 and 2 were newly found in the present study. Zone No. 2 had 3 valiants controlled by a autosomal codominant alleles: Pa-2A and Pa-2B. The gene frequency of Pa-2A and Pa-2B was 0.412 and 0.588, respectively. Zone No. 1 had 3 valiants controlled by 2 autosomal codominant alleles: Pa-3A and Pa-3B. The gene frequency of Pa-3A and Pa-3B was 0.722 and 0.278, respectively.Protein polymorphisms were observed in zone Nos. 1, 2 and 8 in the quail yolk and in zone nos. 1 and 2 in the duck yolk.