Comparative studies of phytochemical analysis and pharmacological activities of wild and micropropagated plant ethanol extracts of Manihot esculenta

Abstract

Abstract The present work was aimed to compare the phytochemical constitutes, antioxidant and cytotoxicity activities of wild and micropropagated Manihot esculenta ethanol (WMEE and MMEE) extracts. M. esculenta nodal regions were used for micropropagation. The explants were subjected to various chemicals for sterilization after that the explant inoculated on Murashige and Skoog (MS) medium supplemented with various concentration and combination of plant growth regulators. In micropropagation of M. esculenta, the plant growth regulators combination 6-benzyl amino purine (8.88 μM) along with naphthalene acidic acid (0.98 μM) and 1.16 μM kinetin (1.16 μM) were observed best shoot formation. Wild and micropropagated plants were extracted by ethanol which extracts performed the phytochemical quantitative screenings, FTIR and GCMS for identification of phytocompounds. The major secondary metabolites were presented in both sample extracts which are confirmed by preliminary phytochemical quantification tests. WMEE and MMEE extracts phytochemical constitutes were identified by FTIR and GC-MS analysis. In FTIR spectrum results, five and two major peaks were reported and 80 to 85 phytocompounds were identified by GCMS. Further, both sample extracts were studied the antioxidant property by various methods and colon carcinoma cell viability through MTT assay. In antioxidant activity, the WMEE and MMEE extracts showed significant activity in hydrogen peroxide scavenging. The cytotoxicity result the WMEE extract was potential in colon carcinoma cell than MMEE extract. From the phytochemical and pharmacological studies, the WMEE and MMEE extracts reported almost similar biological activities. So, the micropropagated plants will be used instead of wild plant for pharmaceutical purpose.