Abstract
The freezing of sperm is a method of conservation of spermatozoa, theoretically for an unlimited time. The freezing methods are an important tool for preservation of genetic diversity of the species and in assisted reproduction. The principle for cryopreservation of semen is reducing temperature of sperm determines a reduction of the metabolic activity of spermatozoa that permits their storage. A number of freezing methods have been described in the literature in the last 30 years. Some methods are registered marks and require buing a francise to be used. Proprietary agencies include Canine Cryobank, CLONE, International Canine Semen Bank (ICSB) and Synbiotics. Nonproprietary systems with published protocols include the Norwegian Tris extender, the Uppsala-Equex system 1 and 2 and its modifications, the CERREC extender, the CERCA extender, the extender developed by England C.G.W. and Lofstedt R. (Concannon and Battista 1989; England 1993; Farstad 1996; Linde- Forsberg et al. 1995; Pena and Linde-Forsberg 2000 a, b) and other extenders and cryopreservation methods.
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