Abstract
A comparison was made of the sizes of the nucleus within epidermal cells from the shed skin of six species of mole salamanders (genus Ambystoma), and three different hybrid complexes including diploid, triploid, and tetraploid animals. The shed skin cells of diploids have smaller nuclei than those of polyploid animals. Nuclei of tetraploid cells are largest but their dimensions overlap those of triploid cells. Nuclei of shed skin cells can be measured to accurately distinguish diploids from polyploids in mixed populations without injury of any kind to the animals.
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