Comparative secretomic and N-glycoproteomic profiling in human MCF-7 breast cancer and HMEpC normal epithelial cell lines using a gel-based strategy.

Aik-Aun Tan,Lik-Voon Kiew,Yeng Chen,Alan Kang-Wai Mu

doi:10.1186/s12935-014-0120-x

Aik-Aun Tan, Lik-Voon Kiew + Show 2 more

Open Access

https://doi.org/10.1186/s12935-014-0120-x

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Abstract

BackgroundConcurrent study of secretomic and glycoproteomic profiles in cancer cell lines represents an excellent approach for investigating cancer progression and identifying novel biomarker candidates. In this study, we performed a comparative secretomic and N-glycoprotein profiling from the secretions of normal human mammary epithelial cells (HMEpC) and the MCF-7 human breast cancer cell line.MethodWe analyzed these cell lines using a combined methodology involving glycan-binding lectins and two-dimensional electrophoresis and identified several differentially secreted factors, including osteonectin and haptoglobin.ResultNotably, comparative analyses also revealed that MCF-7 cells produced differentially N-glycosylated forms of haptoglobin.ConclusionThe present data suggested that osteonectin and haptoglobin might have potential to be served as potential biomarkers for breast cancer. However, further investigation is needed to validate the findings.

Highlights

Breast cancer is one of the most common cancers affecting women worldwide, accounting for almost 23% of all cancers diagnosed in women [1,2]
Comparison of the MCF-7 and human mammary epithelial cells (HMEpC) growth media profiles Separation of MCF-7 media by 2D-E resulted in a highresolution protein profile, which was comprised of various secreted factors (Figure 1B)
A protein cluster was solely detected in HMEpC media but was absent in MCF-7 media

Summary

Introduction

Breast cancer is one of the most common cancers affecting women worldwide, accounting for almost 23% of all cancers diagnosed in women [1,2]. The mortality rate has decreased by 26.58% from 1991 to 2005, breast cancer remains as one of the leading causes of death in women [3] This is mainly due to late stage diagnosis and lack of effective treatments. Human breast cancer cell lines provide an excellent tool for investigating tumor progression and therapy. Comparative studies of N-glycosylation profiles of secreted proteins in malignant cell lines and normal cell lines could provide important clues related to tumor diagnosis and prognosis. Concurrent study of secretomic and glycoproteomic profiles in cancer cell lines represents an excellent approach for investigating cancer progression and identifying novel biomarker candidates. We performed a comparative secretomic and N-glycoprotein profiling from the secretions of normal human mammary epithelial cells (HMEpC) and the MCF-7 human breast cancer cell line

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Discussion

Conclusion