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Abstract
Antibodies to ovine prolactin were used in a radioimmunoassay for prolactin in pituitary extracts from various species of vertebrates. Representative species were chosen from five different classes. The degree of cross-reaction was highest in mammals (62.5%) and lowest in bony fishes (16.2%). Birds, reptiles, and amphibians were intermediary (27·0, 31·9, and 25·0% respectively). In most cases, the immunological relatedness of the prolactins agreed with biological similarities. Prolactin from a few animals (mud puppy, mouse and snake) however, displayed very low immunological reactions. These discrepancies are discussed in relation to different molecular sites for immunological and biological activities.
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