Abstract
Two winter rapeseed cultivars, "NS" (cold tolerant) and "NF" (cold sensitive), were used to reveal the morphological, physiological, and proteomic characteristics in leaves of plants after treatment at -4°C for 12 h(T1) and 24 h(T2), and at room temperature(T0), to understand the molecular mechanisms of cold tolerance. Antioxidant activity and osmotic adjustment ability were higher, and plasma membrane injury was less obvious, in NS than in NF under cold stress. We detected different abundant proteins (DAPs) related to cold tolerance in winter rapeseed through data-independent acquisition (DIA). Compared with NF, A total of 1,235 and 1,543 DAPs were identified in the NSs under T1 and T2, respectively. Compared with NF, 911 proteins were more abundant in NS only after cold treatment. Some of these proteins were related to ROS scavenging through four metabolic pathways: lysine degradation; phenylalanine, tyrosine, and tryptophan; flavonoid biosynthesis; and ubiquinone and other terpenoid-quinone biosynthesis. Analysis of these proteins in the four candidate pathways revealed that they were rapidly accumulated to quickly enhance ROS scavenging and improve the cold tolerance of NS. These proteins were noticeably more abundant during the early stage of cold stress, which was critical for avoiding ROS damage.
Highlights
Cold temperatures have adverse effects on the growth and development of plants [1]
Activity of Superoxide dismutase (SOD), POD, and CAT was used to assess Reactive oxygen species (ROS)-scavenging ability, soluble sugar, and soluble protein as osmotic regulative substances, while MDA content and conductivity were used to assess the degree of peroxidation in plants under cold stress
The activity of SOD, POD, CAT, and the content of soluble sugar and soluble protein was higher in NS than in NF under cold stress
Summary
Cold temperatures have adverse effects on the growth and development of plants [1]. Winter rapeseed (Brassica napus) is one of the few crop species able to survive frosty winters in temperate regions [2]. Only strong cold-tolerant cultivars can survive frigid winters, while cultivars with only slight cold tolerance often wither during winters [3]. Plants originating from temperate regions frequently encounter low temperatures in the early spring and late autumn; perennial or overwintering crops, such as holly [4], winter wheat [5] and winter rape [2], normally experience cold stress while overwintering. Plants have evolved several mechanisms to adapt to low temperatures, including cold-adapted regulation in physiological, biochemical, and molecular processes [6].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
