Abstract
Fusarium oxysporum is a soil-inhabiting fungus that induces vascular wilt and root rot in a variety of plants. F. oxysporum f. sp. conglutinans (Foc), which comprises two races, can cause wilt disease in cabbage. Compared with race 1 (52557−TM, R1), race 2 (58385−TM, R2) exhibits much stronger pathogenicity. Here, we provide the first proteome reference maps for Foc mycelium and conidia and identify 145 proteins with different abundances among the two races. Of these proteins, most of the high-abundance proteins in the R2 mycelium and conidia are involved in carbohydrate, amino acid and ion metabolism, which indicates that these proteins may play important roles in isolate R2’s stronger pathogenicity. The expression levels of 20 typical genes demonstrate similarly altered patterns compared to the proteomic analysis. The protein glucanosyltransferase, which is involved in carbohydrate metabolism, was selected for research. We knocked out the corresponding gene (gas1) and found that Foc-∆gas1 significantly reduced growth rate and virulence compared with wild type isolates. These results deepened our understanding of the proteins related to F. oxysporum pathogenicity in cabbage Fusarium wilt and provided new opportunities to control this disease.
Highlights
Fungi Phaenaerochaete chrysosporium and Lentinula edodes were two of the earliest intracellular filamentous fungi studied using proteomics[13]
Large economic losses for cabbage are caused by the pathogenic fungus F. oxysporum f. sp. conglutinans; little is known regarding the fungus at the protein level
We report the first proteomic map of Foc and provide a comparative analysis overview on the conidia and mycelium between two races of this pathogen using a proteomic strategy
Summary
Fungi Phaenaerochaete chrysosporium and Lentinula edodes were two of the earliest intracellular filamentous fungi studied using proteomics[13]. Discriminating the factors between races that exhibit different pathogenicity towards a specific plant is an essential step to discerning the pathogenic proteins and providing effective disease control measures against F. oxysporum infection. We identified the proteins that exhibited different abundances in the mycelium and conidia among the two races These studies are crucial to understanding the biological and molecular basis of these specialized structures in this fungal pathogen’s life cycle. The studies will aid in identifying the proteins with different abundances that are related to R2’s stronger pathogenicity. Such information may help clarify the pathogenicity mechanism underlying this important pathogen, which will aid in disease control
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