Abstract
Fusarium oxysporum is a soil-born fungus that induces wilt and root rot on a variety of plants. F. oxysporum f. sp. conglutinans (Foc) can cause wilt disease on cabbage. This study showed that a homolog of SIX1 protein in the Arabidopsis infecting isolate Fo5176 (Fo5176-SIX1) had four isoforms in the conidia of Foc by proteomic analysis. Thus, we analyzed the roles of protein Foc-SIX1. Gene expression analysis showed that, compared to the expression in mycelia, dramatically altered expression of Foc-SIX1 could be detected after infecting cabbages, and Foc-SIX1 was highly expressed in conidia under axenic culture condition. Furthermore, we knocked out the Foc-SIX1 gene and found that Foc-ΔSIX1 mutants had significantly reduced virulence compared with wild type isolate, and full virulence was restored by complementation of Foc-ΔSIX1 mutants with Foc-SIX1. Thus, we concluded that SIX1 in Foc was required for full virulence on cabbage. We also complemented Foc-ΔSIX1 with SIX1 gene in F. oxysporum f. sp. lycopersici (Fol) and found Foc-ΔSIX1::Fol-SIX1 mutants did not affect the virulence of Foc-ΔSIX1. The results confirmed that Fol-SIX1 was not capable of replacing the role of Foc-SIX1 in Foc on the disease symptom development of cabbage. The roles of Fol-SIX1 on virulence might rely on host specificity.
Highlights
The soil-born fungus Fusarium oxysporum f. sp. conglutinans (Foc) causes vascular wilt disease of cabbage
A conserved SIX1 homolog was identified in Foc by proteomic analysis
461 protein spots were detected in the conidia of Foc
Summary
The soil-born fungus Fusarium oxysporum f. sp. conglutinans (Foc) causes vascular wilt disease of cabbage. Conglutinans (Foc) causes vascular wilt disease of cabbage. Fusarium wilt of cabbage originated in the United States and was first reported in China in 2001 [1]. Lycopersici (Fol) and tomato has become a model system for studying the effectors of the pathogen and R proteins from the host [2]. The secreted-in-xylem (SIX) proteins were first reported in the xylem sap of Fol-infected tomato [3]. Eleven SIX proteins have been identified in Fol so far, and the functions of most of these proteins remain unknown [2].
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