Abstract
Background and objectivesThe variation in the active phytoconstituents in different plants causes changes in their biological properties. The present study was aimed with the comparative phytochemicals and estimation of the constituent present in the leaves of Decalepis species using high performance liquid chromatography (HPLC). Materials and MethodsThe leaves of Decalepis hemiltonii Wight & Arn. Decalepis arayalpathra(J. Joseph and V. Chandras.) Venter. and Decalepis nervosa(Wight &Arn.) Venter.were selected and methanol solvent was used for the preparation of the extracts. Various proximate analysis viz. moisture content, ash values, crude fibers, extractive values were estimated. Thereafter, series of basic chemical tests for the methanol leaves extracts followed by total phenolics, and total flavonoids content were also carried out. Furthermore, TLC chromatogram and RP-HPLC study was carried out for all the plant extracts to find out the presence of phenolic compounds and compared the estimated amount present in all the leaves extracts. ResultsThe outcome of the study revealed that all the three species varied with the yield and the phytochemical screening showed varied concentration of alkaloids, glycosides, flavonoids, proteins, steroids, phenols, and tannins. Among the three species Decalepis hemiltonii(DH) showed presence of higher percentage of moisture (8.6%), crude fibers (8.32%) and total ash content (4.95%) than others. Further, presence of high concentration of phytochemicals was showed by Decalepis arayalpathra (DA) and the higher yield in methanol solvent than other two species. The same plant sample showed presence of higher amount of gallic acid (0.86 mg/100 g) than other two species. Total alkaloids, total phenolic and total flavonoids were higher in DA leaves flowed by Decalepis nervosa (DN) and DH leaves samples. InterpretationOverall the results indicated that DA leaves extracts was better in terms of yield, presence of phytoconstituents, presence of total alkaloids, total flavonoids, and total phenolics and presence of gallic acid followed by DN and DH leaves.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
