Abstract
Preeclampsia is a serious complication of pregnancy, which affects 2–8% of all pregnancies and is one of the leading causes of maternal and perinatal mortality and morbidity worldwide. To better understand the molecular mechanisms involved in pathological development of placenta in preeclampsia, we used high-resolution LC-MS/MS technologies to construct a comparative N-glycoproteomic and phosphoproteomic profiling of human placental plasma membrane in normal and preeclamptic pregnancies. A total of 1027 N-glyco- and 2094 phospho- sites were detected in human placental plasma membrane, and 5 N-glyco- and 38 phospho- proteins, respectively, with differentially expression were definitively identified between control and preeclamptic placental plasma membrane. Further bioinformatics analysis indicated that these differentially expressed proteins correlate with several specific cellular processes occurring during pathological changes of preeclamptic placental plasma membrane.
Highlights
The mechanisms involved in maintaining a human pregnancy, or the switches between the normal pregnancy outcome and adverse outcome such as miscarriage, preeclampsia, fetal growth restriction, or preterm labor, are complicated
We confirmed that the representative pathological changes of placenta Plasma membrane (PM) between normal and preeclamptic pregnancies were consistent with those previously reported [18,19]
By the large-scale proteomic analysis, we identified 1027 N-glyco- and 2094 phospho- sites in the human placental PM with high confidence
Summary
The mechanisms involved in maintaining a human pregnancy, or the switches between the normal pregnancy outcome and adverse outcome such as miscarriage, preeclampsia, fetal growth restriction, or preterm labor, are complicated. They become widely dilated and lose their responsiveness to vasoconstrictive stimuli. Preeclampsia (PE) affects about 2 to 3% of all pregnancies but this number can be even higher in underdeveloped countries It is an important cause of maternal death worldwide and a leading cause of iatrogenic prematurity and fetal growth restriction. The maternal syndrome is, at least in part, caused by the maternal response to this placenta damage. This is known as the two-stage model of preeclampsia [4,5]
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