Comparative morphometry of Bergmann glial (Golgi epithelial) cells

Abstract

Bergmann glial (Golgi epithelial) cells were Golgi-impregnated in the cerebella of species with great differences in the thickness of the molecular layer, in small African native mouse, rat, rhesus monkey, and man. The thickness of the molecular layer determines the length of the radial Bergmann cell processes. Whereas the overall morphology of the cells was found to be strikingly similar in all species studied, there were great quantitative differences in length and diameter of the stem processes. Species with thick molecular layers (man, monkey) have thicker stem processes than species with short distances between Bergmann glial cell soma and pial surface (rat, mouse). This could mean that larger animals with longer gestation periods allow for prolonged growth of cell volumes. On the other hand, an increase in the diameter of long processes should reduce the cytoplasmic resistance against ionic currents; this would be important when Bergmann glial cells--like retinal Müller cells--would act as "cables" for spatial buffering of potassium ions released by electrically active neurons. By contrast, the fractal dimension--i.e., a quantitative measure of the complexity of the cell's border--of the cell processes was lower in species with long processes. In an age series of rat cells, the fractal dimension is shown to increase slightly up to a very old age.