Comparative inactivation of Aujeszky's disease virus, Porcine teschovirus and Vesicular stomatitis I by chemical disinfectants

Abstract

We tested the germicide activity of 1% Chloramin BM, 1% Incidin Plus, 1% Lysoformin 3000, 0.2% Mikasept KP, and 2% Sekusept Forte against viruses in suspension (suspension test) and dried onto a surface (carrier test). The agents of the porcine encephalomyelitis (<I>Porcine teschovirus</I>, strains CAPM V-86, CAPM V-37), Aujeszky’s disease (strains CAPM V-166, CAPM V-327) and vesicular stomatitis (strains CAPM V-499, CAPM V-331) were used as model viruses. After 30 min contact time in both the suspension and carrier tests, the <I>Porcine teschovirus</I> was 4 lg inactivated only by Mikasept, which was thus the only disinfectant to meet the standard. The other disinfectants decreased the viral titre insufficiently. Under the same conditions, <I>Aujeszky’s disease virus</I> was inactivated by at least 4 lg by all the tested disinfectants except for Chloramin BM which decreased the titre of CAPM V-166 only by 3.75 lg in the carrier test. For the inactivation of <I>Vesicular stomatitis virus </I>Chloramin BM and Mikasept KP were tested. Both the disinfectants reliably decreased the viral titre in both the suspension and carrier tests. Our results show that the inactivation of a surface-bound virus is more difficult than its inactivation in suspension. We confirm the high resistance of non-enveloped viruses (<I>Porcine teschovirus</I>) to chemical inactivation.