Comparative in vitro antimicrobial study of Panchavalkal and modified Panchavalkal in three different extract solvents: a study protocol

Abstract

Background: Charak, Sharangdhar, Kashyap, BhavaPrakasha described Panchavalkala, is a combination of five astringent barks viz., Vata (Ficus bengaenesis Linn.), Udumbara (Ficus glomerata Roxb.), Ashvattha (Ficus religiosa Linn.), Parisha (Thespesia populanea Soland excorrea) and Plaksha (Ficus slacor Buch-Ham.). In Ayurveda sometimes substitute plants are described to work better for a particular medical condition. Such type of alternative drug suggested by BhavaMishra and Kaiyadeva Nighantu for classical Panchavalkala is Shirish (Albezzia lebbeck Linn.) in place of Parisha. Such type of altered Panchavalkal is called as modified Panchavalkal. Both Panchavalkals are essentially important in acute, chronic ulcers, wound healing and reproductive health concern women’s diseases like leucorrhea, irregular or heavy bleeding etc. So, these Panchavalkals at three different extract solvents i.e., water extract, alcoholic extract and hydro alcoholic extract in comparison to standard anti-microbial drugs will be studied.Methods: Panchavalkal and modified Panchavalkal in water, alcohol and hydro alcoholic extract will be subjected to a test antimicrobial property by agar well diffusion method. Results will be assessed on the basis of minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and the zone of inhibition (ZOI) of plant extracts against the microorganism that will be measured.Conclusions: Conclusion will be drawn on the basis of results obtained about anti-microbial activity of Pachavalkal and modified Panchavalkal in three different extracts.Trial registration: It is taken, Ref. No. MGACHRC/IEC/February-2021/193-A