Abstract
The results involved in the histochemical technique for the detection of sodium(Na) ion have been analysed. Nonspecific precipitation occurs when potassium(K) antimonate reacts with the ethanol used for dehydration, and also may occur when the pH is below 6.8 during fixation. Since calcium(Ca) and magnesium(Mg) ions can form precipitates with antimonate(Sb) there is a possibility of interference by Ca and Mg ions in tissues. There nonspecific reactions must not be confused with the specific histochemical reaction. The K-phosphate and collidine buffer used to control the pH seems to inhibit the precipitation reaction of K-antimonate with Na ion. The reduced formation of precipitate with K in preserve of these buffers might be caused by an altered ionic balance between the dissociated antimonate and other correlating ions, such as K, H( pH below 6.8 ), or collidine. The non-buffered fixative, however, seems to good results at the electron microscopic observation. On the other hand, Legato et al reported on the Ca-ion localization in the cardiac muscle using the same method for the Na-detection.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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