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Abstract
Summary Exfoliated epithelial cells from nasopharynx of 27 individuals were collected with a low-negative-pressure suction apparatus. These cells were examined cytologically and stained for Epstein-Barr virus (EBV) nuclear antigen on touch smears by immunoperoxidase. DNA was extracted from the remaining cells and studied for the presence of EBV sequences by blot and spot hybridization. In the 22 positive nasopharyngeal carcinoma cases (confirmed by clinical, histological and cytological examination) EBV DNA was found with both techniques in 21 cases, while in the 5 cases of chronic inflammation without tumorous cells, no EBV DNA was detected.
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