Comparative Evaluation of Two Different Novel Formulations of Quercetin Against Non Melanoma Skin Cancer in Human Subjects

Abstract

Nonmelanoma skin cancer (NMSC) is the steadily increasing cancer in the India. Various studies have put forward strong evidences of NMSC incidence due to UV radiation exposure. Keratinocyte macrophage cells express macrophage which represents both type of receptor viz. folate binding receptor having affinity for folic acid and mannose-binding receptor which are having affinity towards mannose. This study was designed to identify the correlation parameter in evaluation of in vivo doses and epidermal changes following UVB irradiation using noninvasive techniques. We in our study have developed two different formulations embedded with endogenous ligands conjugated nanoparticles of same drug (quercetin) and have analyzed its effect on human volunteers against the release of MIF and MED analysis for which prior permission from Human Ethical Committee has been obtained accordingly. The result of the study showed that skin biopsies sections of human subjects showed that there was a dose dependent effect on human skin in response to the UV radiations. The pattern was nearly same for NL-4C and NL-5C for MED analysis. While for MIF release study, for first 12 hours the MIF level ranged from 12.34 ± 9.90 ng/ml to 79.4 ± 23.65 ng/ml which was the result of acute exposure of UV radiations. For next 24-48 hours, the MIF levels was found to be increased from 22.43 ± 12.56 ng/ml to 102.34 ± 20.4 ng/ml which shows that the prepared formulations were significant in inhibiting the release of MIF.