Abstract
BackgroundAkabane disease (AD), a barrier to international trade for endemic areas with far economic impact on the countries, is caused by Akabane virus (AKAV). Commercial enzyme-linked immunosorbent assay (ELISA) is a commonly used diagnostic technique for AKAV infection, including the IDEXX and IDVET ELISA kits. However, the comparative evaluation of the IDEXX and IDVET ELISA kits has not been published. The object of this study was to evaluate the test performance of the two commercial ELISA kits in detecting serum anti-AKAV antibodies in cattle.ResultsWith virus neutralization test (VNT) as the “relative gold standard”, the diagnostic sensitivity (DSe) was 80.39% (123/153) and 93.46% (143/153) for the IDEXX and IDVET ELISA kit, when suspect samples were included. The diagnostic specificity (DSp) for the IDEXX and IDVET ELISA kit was 93.48% (502/537) and 82.31% (442/537), respectively.ConclusionBoth of the tested ELISA kits could be applied to detect antibodies against AKAV in cattle serum. The IDVET ELISA kit had a higher DSe. The IDEXX ELISA kit possessed the higher DSp. These results have important implications if the kits are used to screen herds or individual cattle in surveillance programs, or at border crossings for import-export inspection and quarantine.
Highlights
Akabane disease (AD), a barrier to international trade for endemic areas with far economic impact on the countries, is caused by Akabane virus (AKAV)
AKAV is a member of the Simbu serogroup belonging to the genus Orthobunyavirus in the family Peribunyaviridae [4,5,6], which includes Schmallenberg virus (SBV) [7], Shamonda virus (SHAV) [8], Douglas virus (DOUV) [9] and Sathuperi virus (SATV) [10]
Diagnosis of infections caused by AKAV is traditionally accomplished by detection of specific antibodies through virus neutralization test (VNT), and if necessary, the virus is identified by virus isolation
Summary
Akabane disease (AD), a barrier to international trade for endemic areas with far economic impact on the countries, is caused by Akabane virus (AKAV). The object of this study was to evaluate the test performance of the two commercial ELISA kits in detecting serum anti-AKAV antibodies in cattle. AKAV is Diagnosis of infections caused by AKAV is traditionally accomplished by detection of specific antibodies through virus neutralization test (VNT), and if necessary, the virus is identified by virus isolation. These techniques are labor-intensive, time-consuming and difficult to implement for large numbers of samples [18]. Several commercial ELISAs have been developed to detect antibodies against AKAV, which are ready-to-use and can be applied to large-scale screening and serological
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