Abstract
Seven regenerable oat genotypes were cultured via callus, multiple meristem and multiple meristem-to-callus combinations to produce plants. Meristem-to-callus refers to the induction of multiple meristems under reduced auxin followed by the initiation and proliferation of callus from such multiple meristems under high auxin-containing media. Numbers of regenerated plants from all three tissue culture methods were significantly higher (P=0.05) in GP-1 than in other genotypes. In the GP-1 x Corbit and Corbit x GP-1 genotypes, higher plant numbers were observed when GP-1 was the maternal parent. For Corbit, an agronomically important oat genotype, tissue culture methods using callus and meristem-to-callus produced more plants per gram than using multiple meristem cultures alone. The meristem-to-callus method, in particular, produced substantial numbers of plants per gram in Corbit.
Published Version
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