Comparative Evaluation of Direct Smear and Culture Methods for Detection of Trichomonas gallinae Infection in Pigeon and Chicken of Assam

Abstract

A study was carried out in order to compare the detection of Trichomonas gallinae by examination of smears of throat swab and culture of protozoa collected from both pigeon and chicken. In the present study, five culture media viz. Modified Diamond’s Media, Nutrient broth, Medium 199, Minimum Essential Medium (MEM) and Roswell Park Memorial Institute-1640 (RPMI-1640) were used for culture of T. gallinae. Microscopic examination of wet mount/ Giemsa stained smear and culture of the T. gallinae revealed the latter method to be superior to wet mount preparation or staining methods.