Abstract
Introduction : microscopy is the backbone of traditional malaria diagnostics. However, availability of technical assistants on military deployments who are well experienced with malaria microscopy is scarce. Therefore, molecular diagnostic procedures are desirable which are widely independent from the skills of the investigator. Methods : in-house and commercial PCRs systems and various nucleic acid extraction procedures were compared. Results : there was no relevant difference in the performance of a well-established in-house PCR approach in comparison with a commercial PCR kit for the detection of malaria. Automated nucleic acid extraction from blood samples proved to be as reliable as manual column-based nucleic acid extraction. Conclusion : the results demonstrate the basic suitability of molecular diagnostic approaches for the detection of malaria with a high degree of automation for the deployment setting. Potential future optimization may include modern approaches like loop-mediated amplification (LAMP), which is currently under investigation at our department.
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