Comparative Evaluation of Antimicrobial activity of Tulsi extract [Ocimum Sanctum (Linn.)] and Curcumin extract [Curcuma Longa] with 0.2% Chlorhexidine on Periodontal Pathogens

Abstract

BACKGROUND: Periodontal disease is associated with colonization of specific bacteria in the subgingival area. Widespread use of antimicrobials has resulted in emergence of various side effects and drug resistance. Phytomedicine has been used in many clinical conditions and it appears to be a suitable alternative to manage conditions affecting the oral cavity. AIM: Present study aims to assess the in-vitro antimicrobial efficacy of Tulsi extract and Curcumin extract on Periodontal pathogens. MATERIALS & METHODS: Ethanolic extract of 100% w/v of Tulsi and Curcumin was prepared by cold extraction technique. Prepared extracts were further diluted to obtain solutions of 5 different concentrations to be tested (0.5%, 1%, 2% and 5%). Obtained solutions and controls (0.2% Chlorhexidine and Distilled water) were subjected to the microbiological investigation against Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. Agar well diffusion method was employed to determine the concentration at which Tulsi and Curcumin gave an inhibition zone, similar to Chlorhexidine. Data was analysed using one‑way analysis of variance and post‑hoc test for inter-group and intra‑group comparisons. RESULTS: At 10% concentration both Tulsi and Curcumin extract have demonstrated comparable antimicrobial activity against Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis.