Comparative effects of sodium hydrosulfide and proline on functional repair in rice chloroplast through the D1 protein and thioredoxin system under simulated thiocyanate pollution

Abstract

Various environmental contaminants can find their way to enter plant cells and disturb and/or damage the essential components of PSII repair cycle in chloroplast, thereby resulting in dysfunction of chloroplast. In the current research, a microcosm hydroponic experiment was set up to evaluate the comparative effects of sodium hydrosulfide (NaHS)- and proline (Pro)-mediated functional repairing of chloroplast in rice plants under SCN− stress. Our results displayed that when exposed to environmental realistic SCN− concentrations (24–300 mg L−1), foist significant (p < 0.05) gene-dose repercussion on the pathways of photosynthetic reactions and energy metabolism in rice shoots, and a downturn in the level of total soluble starch, sugar, and chlorophyll. Sodium hydrosulfide application effectively mitigated (p < 0.05) the toxic effects of SCN− in rice seedlings by stimulating the processes of phosphorylation, dephosphorylation and new-synthesis of D1 protein in the PSII repair cycle, and increased the turnover of D1 protein to recover CO2 assimilation. Evidently, Pro treatment mainly enhanced (p < 0.05) the expression of magnesium chelatase (MgCh) and ribulose-1,5-bisphosphate carboxylase (Rubisco) related genes under simulated SCN− stress, suggesting that the targeted repairing site in chloroplast by Pro was different from NaHS. The outcome of the present research contributes to a better understanding at molecular level for repairing of chloroplast functional disorder by NaHS and Pro at different key nodes under SCN− stress.