Abstract
An alternative approach to the separation of hydrophobic compounds by electrokinetic chromatography using a negatively charged cyclodextrin, sulfobutyl ether-β-cyclodextrin (SBE-β-CD), as a pseudostationary phase is described. While the separation power of SBE-β-CD is comparable with sodium dodecyl sulfate for compounds with relatively low retention factors, a considerable improvement in resolution and small differences in selectivity were observed with this pseudostationary phase for highly hydrophobic compounds exemplified by C 5–C 11 phenones. Moreover this approach allows electrokinetic chromatography to be performed in reversed flow mode where the pseudostationary phase elutes in the opposite direction to the electroosmotic flow, even at neutral pH and without the need for treated capillaries or addition of organic modifiers, thereby expanding the elution range.
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