Abstract
Burkholderia pseudomallei and Burkholderia mallei are the causative agents of melioidosis and glanders, dangerous infectious diseases. These microorganisms are not endemic for the central zone of the Russian Federation, but the possibility of an imported case or deliberate use as a biological weapon is not ruled out. In practical work for accelerated detection and subsequent identification of pathogenic microorganisms, the method of orienting reaction of slide-agglutination is most often used as the simplest, requiring no additional equipment and easy enough to perform. Since in Russia standard (production) diagnostic species-specific sera are not produced for any Burkholderia species, experimental series of preparations are used in the conditions of specialized laboratories for setting diagnostic immunological reactions. In the present work we investigated four types of experimental rabbit immune serum using strains of pathogens melioidosis and glanders collection of Volgograd Research Plague Control Institute. The most active was serum obtained against live B. pseudomallei VPA cells, the least is serum against B. pseudomallei C-141; with complex serum against cells of 5 strains of Burkholderia thailandensis and with serum against extracellular antigen B. thailandensis 264 agglutination was observed only at low dilutions.
Highlights
FPHI «Volgograd Research Plague Control Institute» of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare
Burkholderia pseudomallei and Burkholderia mallei are the causative agents of melioidosis and glanders, dangerous infectious diseases
In the present work we investigated four types of experimental rabbit immune serum using strains of pathogens melioidosis and glanders collection of Volgograd Research Plague Control Institute
Summary
FPHI «Volgograd Research Plague Control Institute» of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare. Burkholderia pseudomallei and Burkholderia mallei are the causative agents of melioidosis and glanders, dangerous infectious diseases. These microorganisms are not endemic for the central zone of the Russian Federation, but the possibility of an imported case or deliberate use as a biological weapon is not ruled out. In the present work we investigated four types of experimental rabbit immune serum using strains of pathogens melioidosis and glanders collection of Volgograd Research Plague Control Institute. Возбудители мелиоидоза (Burkholderia pseudomallei) и сапа (Burkholderia mallei) относятся к микроорганизмам II группы патогенности (опасности). Pseudomallei и B. mallei неоднороден, зависит, как правило, от географической принадлежности штаммов и обуславливает результаты агглютинации различными сыворотками. Именно данный факт объясняет отрицательные результаты агглютинации выделенного изолята [6]
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