Abstract
Here, we performed a comparative evaluation of the effectiveness of the isolation of human interferon-gamma from the biomass of the recombinant strain of E.coli MH-1/pIFN-γ-trp2 enabling constitutive synthesis of the target protein in the "inclusion bodies", and a new recombinant inducible strain of E. coli BL 21/pET-IFN-γ containing the protein in a soluble form. It has been shown that the use of an inducible producer strain can increase the yield of the target protein from 1.5 mg/g to 5 mg/g and enhance the specific (antiviral) activity by 20-fold.
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