COMPARATIVE CHARACTERISTIC OF PROLIFERATION OF FIBROBLASTSAND FUNCTIONAL ACTIVITY OF MAST CELLS IN REGENERATION HISTOGENESISAFTER A GUNSHOT WOUND

Abstract

Objective. To give a comparative description of the functional activity of mast cells and the proliferation of fibroblasts in regenerative histogenesis after gunshot damage. Materials and methods. The material for the study was histological preparations, obtained after a gunshot wound in the fold of the rat's back skin with a shot at the stop from the Margolin pistol with a 5.6 mm bullet. Taking the material was carried out 6, 24 hours, 3, 6, 15 and 25 days from the beginning of the experience (3 animals for each term). Control group (without injury) - 3 animals. Methods of research - light microscopy, histoautoradiography method, quantitative analysis and statistical processing of data. Fat cells and proliferating cells of the fibroblast differon in the perinecrotic region were studied, taking into account histotopography. Resullts of the study. An immediate response to injury is active degranulation of mast cells (MC), recorded 6 hours after the damage. For the first time, proliferating cells of fibroblastic differential are observed in the deeper layers of the skin on the second day. Regenerative histogenesis of connective tissue and the formation of granulation tissue occurs from 3 to 15 days from cambial sources of the connective tissue “case” of the skin muscle. MC degranulation is observed throughout the observation period. By day 25, the largest number of MC and fibroblasts, includingH3-thymidine, was recorded in subepithelial loose connective tissue. Conclusion. Quantitative, topographical, temporal characteristic of population change and localization of MC in comparative aspect with the same criteria in relation to proliferating cells of fibrooblast differon shows the participation of biologically active substances in attracting the precursors of fibroblasts to the wound surface and regulating their proliferation during the wound histogenesis (4 f igs, bibl iography: 5 refs).