Abstract
Aim - to study the reparative regeneration processes after experimental gradual traction of cervix uteri in rats. Materials and methods. The object of the study is the cervix at the sphincter. We performed gradual traction of cervix uteri in 25 rats under aether anesthesia and gathered the samples.We analyzed the course of reparative regeneration and performed sampling after 1, 3, 5, 7, 10, 15, 21 and 30 days. We carried out complex morphological examination with optical and transmission electron microscopy, and immunohistochemistry. The immunohistochemical examination of cervical tissues was performed using a set of monoclonal antibodies to а-smooth muscle actin. Results. It was determined that cervical traction formed three distinguishable zones: the zone of damage, the periwound zone and the zone of morphological integrity. The zone of damage corresponds to the actual sphincter and the periwound zone - to pre- and postsphincteral compartments. Several myocytes undergo necrosis in the damage zone. There is no necrosis in the periwound area, but we found some abnormality of intercellular contacts due to the change of the morphology of intercellular substance. The experimental traction causes the development of local septic inflammation, affecting all the layers. The synthesis of fibrous connective tissue components starts in the proliferative phase. It leads to restructuring of histoarchitecture and disruption of the functional syncytium structure. Conclusion. Post-traumatic regeneration is performed by changing the phenotype of survived myocytes from mantle to mantle-synthetic myocytes with synthesis of the fiber component of the intercellular substance. Also, there is an incomplete mitosis in several myocytes, which leads to the formation of binuclear or polyploid cells.
Highlights
Цель —проследить динамику течения репаративной реге нерации в шейке матки крыс при ее повреждении в условиях экспериментального дозированного растяжения
We performed gradual traction of cervix uteri in 25 rats under aether anesthesia and gathered the samples.We analyzed the course of reparative regeneration and performed sampling after 1, 3, 5, 7, 10, 15, 21 and 30 days
Post-traumatic regeneration is performed by changing the phenotype of survived myocytes from mantle to m antle-synthetic myocytes with synthesis of the fiber component of the intercellular substance
Summary
Цель —проследить динамику течения репаративной реге нерации в шейке матки крыс при ее повреждении в условиях экспериментального дозированного растяжения. Объектом исследования служила шейка матки на уровне сфинктера. Взятие материала осу ществляли от 25 половозрелых нерожавших лабораторных крыс, которым под эфирным наркозом выполнено дозиро ванное растяжение шейки матки. Установлено, что растяжение шейки матки приводит к появлению участка, где различимы три зоны: зона повреждения, прираневая зона и зона морфологической целостности. При этом зона повреждения максимально соот ветствует собственно сфинктеру, а прираневая зона —предсфинктерному и постсфинктерному компартментам. В зоне повреждения отдельные миоциты подвергаются некрозу. В прираневой зоне некроза клеток не наблюдается, но заметно нарушение межклеточных контактов за счет изменения мор фологии межклеточного вещества. Посттравматическая регенерация осущест вляется путем смены фенотипа сохранившихся миоцитов с сократительных на сократительно-синтетические и синтезом волокнистого компонента межклеточного вещества. Ключевые слова: шейка матки, миометрий, гладкие миоциты, коллаген, восстановление
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
