Comparative Anti-Inflammatory Activity of Rutin-Based Mouthwash and Diclofenac Sodium: An in-Vitro Evaluation

Abstract

Background: One of the most common oral inflammatory conditions is Gingivitis. It involves the inflammation of gingiva supporting the tooth. Onset of gingivitis is due to the recurrent deposition of plaque and its inefficient removal. Use the right oral dentifrice and mouthwash to keep the inflammation at bay. Rutin is a natural substance extracted from citrus fruits. This citrus flavonoid is known to possess antimicrobial, antioxidant, and cytotoxic properties. Aim: This study aims to formulate a mouthwash with rutin and compare its anti-inflammatory potential with an already commercially anti-inflammatory agent. Materials and Methods: For this in-vitro study two different anti-inflammatory assays were performed using different substrates. The first test performed was the bovine serum albumin anti-inflammatory test and the second one was the egg albumin denaturation test. Results: The results suggest that the rutin mouthwash made in our study shows a similar anti-inflammatory activity as the Diclofenac sodium based formulation. Rutin mouthwash and the Diclofenac sodium have exhibited a non-significance in the percent protein anti-denaturation with the increase in the concentrations ranging from to . Conclusion: Rutin-based mouthwash possesses satisfactory anti-inflammatory activity compared to standard commercial mouthwash.