Abstract
Exploration ofthe abnormal expression ofexosomal molecules during the infection ofavian leukosis virus subgroupJ (ALV-J) isessential toprovide adeeper understanding ofthe exosome's role inthe viral pathogenesis involved. The study aimed toinvestigate the differentially expressed proteins and miRNAs ofthe exosomes derived from DF-1 cells infected byALV-J, their gene function and involved signal pathways. Weisolated exosomesfrom DF-1 cells infected byALV-J. The differentially expressed proteins and miRNAs ofthe exosomes were determined byproteomics and transcription detection technology. AGene Ontology (GO) analysis and aKyoto Encyclopedia ofGenes and Genomes (KEGG) signal pathway analysis identified the miRNAs target genes and thesignal pathways regulated bythe different proteins or/and miRNAs. Atotal of116proteins (58upregulated and 58downregulated) and 3miRNAs (all upregulated) were determined. These proteins were involved in155signal pathways, inwhich the highest number ofproteins involved inthe cancer pathway was (up to) seven. The target genes ofthemiRNAs were involved in3signal pathways. Both the proteins and target genes ofthe miRNAs were involved inthe Ribosome pathway and ECM-receptor interaction pathway. The results suggested that the ALV-J infection changed the proteins and miRNAs ofthe exosomes significantly.
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