Abstract
Relevance. Standardization of plant raw material derived from stevia is a topical issue, since this plant (Stevia rebaudiana Bertoni) contains sweet diterpene glycosides and is used as a natural sugar substitute. In addition, stevia contains flavonoids which are biologically active compounds that exert antioxidant, anti-inflammatory and capillary-strengthening properties. Currently, spectrophotometric methods are used to identify and quantify flavonoids in medicinal plants. They are fast, convenient and do not require sophisticated equipment.
 The aim of our paper is comparative phytochemical study of the content of the total flavonoids in stevia raw material of different origin.
 Materials and methods. The study material was dried stevia leaves (Stevia rebaudiana Bertoni), grown in the Penza Region, the Republic of Crimea, the Krasnodar territory, and raw materials imported from Paraguay and India. The content of the total flavonoids was estimated by differential spectrophotometry calculated with reference to cynaroside.
 Results. The study of the quantity of flavonoids in the raw material of stevia of different origin was carried out. In the presence of aluminum chloride stevia flavonoids form a complex compound at the absorption maximum of 408 2 nm. The content of flavonoids in different species of stevia are found to range from 1.13% to 1.74%.
 Conclusion. The obtained results allow to recommend stevia leaves as a source of flavonoids along with other available medicinal plants. It is advisable to classify the complex of flavonoids as the second group of biologically active compounds of stevia leaves.
Highlights
The study material was dried stevia leaves (Stevia rebaudiana Bertoni), grown in the Penza Region, the Republic of Crimea, the Krasnodar territory, and raw materials imported from Paraguay and India
The content of the total flavonoids was estimated by differential spectrophotometry calculated with reference to cynaroside
In the presence of aluminum chloride stevia flavonoids form a complex compound at the absorption maximum of 408 ± 2 nm
Summary
The study material was dried stevia leaves (Stevia rebaudiana Bertoni), grown in the Penza Region, the Republic of Crimea, the Krasnodar territory, and raw materials imported from Paraguay and India. The obtained results allow to recommend stevia leaves as a source of flavonoids along with other available medicinal plants. It is advisable to classify the complex of flavonoids as the second group of biologically active compounds of stevia leaves. ■ Keywords: Stevia rebaudiana Bertoni; leaves; flavonoids; spectrophotometry; quantitative determination; cynaroside. Проблема стандартизации растительного сырья на основе стевии является достаточно актуальной, так как это растение (Stevia rebaudiana Bertoni) содержит сладкие дитерпеновые гликозиды и используется как натуральный заменитель сахара. Стевия содержит ряд других биологически активных соединений: флавоноиды, сапонины, органические кислоты, каротиноиды [9, 10, 13, 18]. Отмечается, что стевия стимулирует секрецию инсулина и снижает уровень глюкозы в крови. Цель работы — проведение сравнительного фитохимического исследования содержания суммы флавоноидов в сырье стевии различного происхождения
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