Comparative analysis of methods to stimulate and collect dentinal fluid: a clinical and experimental study

Abstract

The aim - to determine the most effective method of dentinal fluid collection and dentinal fluid flow rate stimulation. In a series of dentinal fluid collection tests, 3 types of filter membranes «Technofilter» were compared to a blotting nitrocellulose membrane Sartorius 1288. An ability of the studied membranes to adsorb dentinal fluid was assessed based on the data of proton magnetic resonance using signal magnitudes of the organic molecules accumulation in dentine fluid samples. In the study 30 permanent teeth with a diagnosis of K04.00 «Initial pulpitis» were included. Teeth were randomly divided into 5 groups in accordance with the method used to stimulate the dentinal fluid flow rate. Dentinal fluid volume before and after stimulation within and between the groups was compared. The nitrocellulose membrane Sartorius 1288 has the highest ability to adsorb dentinal fluid; predominant dentinal fluid volume was obtained using a 10% dextran solution with low molecular weight. Based on the results of the study, the author proposed the method of dentinal fluid collection. The proposed stimulation method increases the dentinal fluid yields by 2 times in comparison with sampling under natural conditions.