Comparation of the phytotoxicity between chemically and green synthesized silver nanoparticles

Abstract

Green synthesis of silver nanoparticles (Ag NPs) by using plants extracts has provided an eco-friendly alternation for industry and agriculture application. Here, we prepared Ag NPs by using the cucumber leaves and rice husk extracts, and further assessed the antimicrobial activity and phytotoxicity of green synthesized Ag NPs (g-Ag NPs) comparing with chemically synthesized Ag NPs (chem-Ag NPs). The chem-Ag NPs had strong antibacterial activity on the growth of Escherichia coli, while g-Ag NPs by rice husks (gr-Ag NPs) exhibited long-term antibacterial effects. In terms of phytotoxicity, the chem-Ag NPs induced over-generation of ROS and activated plant antioxidant defense systems, thus resulting in the upregulation of MDA and Zn contents and downregulation of antioxidant capacity, carotenoid, globulin and Mo contents. However, g-Ag NPs significantly promoted cucumber photosynthesis by increasing chlorophyll contents. Besides, the green synthesized Ag NPs by cucumber extracts (gc-Ag NPs) increased protein contents and gr-Ag NPs stimulated the upregulation of Mn and the downregulation of Al, which were all positive effects. Overall, compared with chem-Ag NPs, g-Ag NPs exhibited long-tern antimicrobial properties and attenuated toxicity to plants, which could be used as potential nanopesticide or nanoscale growth regulator in agriculture.