Community Effect on Drug Sensitivity of Cardiomyocytes Controlled Spatial Patterns by using On-Chip MEA System

Abstract

In cardiomyocyte network, network size and spatial arrangement of different cellular-type are important factors for stabilization of beating rhythms. To study the community effect of the geometric factor and community size, we tried to develop cultivation chambers in a various size and geometric patterns using the agarose micro-processing technique combined with the multi-electrode array (MEA) measurement system (we call it On-Chip MEA system), and each of extra-cellular signal of mouse embryonic cardiomyocytes to tachyrhythmia inducing drug response was recorded simultaneously.Firstly, to evaluate the effect of community size on the sensitivity to drug, we tried to build the square sheet type chambers having small, medium and large area (about 104, 105, and 106 um2, respectively). Next, we tried to construct circuit type chambers with loop structure (circuit length of 2um, 8.2um) compared with sheet type geometry.As a result, we observed different response to drug among community size and geometric pattern. These results imply that sensitivity to the drug depends on spatial patterns. In this meeting, difference of drug sensitive event (e.g. tachyrhthmia, cardiac arrest etc), we report in detail about quantitative parameters: Beating Rate (BR), Field Potential Duration (FPD), Short Term Variability (STV) of them etc, which will be help for understanding community effect.