Commonly Used Reagents

Abstract

Abstract Recipes Acid, concentrated stock solutions Acid precipitation solution Alsever's solution Ammonium hydroxide, concentrated stock solution Ammonium acetate, 10 M Ammonium sulfate, saturated ATP, 100 mM Base, concentrated stock solutions BBS (BES‐buffered solution), 2× BCIP, 5% (w/v) BSA (bovine serum albumin), 10% (w/v) CaCl2, 1 M Carbonate buffer Denhardt solution, 100× DEPC (diethylpyrocarbonate)‐treated solutions DNase I, RNase‐free (1 mg/ml) dNTPs: dATP, dTTP, dCTP, and dGTP DPBS (Dulbecco's phosphate‐buffered saline) DTT (dithiothreitol), 1 M EDTA (ethylenediaminetetraacetic acid), 0.5 M (pH 8.0) Ethidium bromide staining solution FBS (fetal bovine serum) FBS, heat inactivated Formamide loading buffer, 2× HBSS (Hanks' balanced salt solution) HCl, 1 M HeBS (HEPES‐buffered saline) solution, 2× KCl, 1 M 2‐ME (2‐mercaptoethanol), 50 mM MgCl2, 1 M MgSO4, 1 M MOPS buffer NaCl, 5 M NaOH, 10 M PCR amplification buffer, 10× Phenol, buffered Phenol/chloroform/isoamyl alcohol, 25:24:1 (v/v/v) Phosphate‐buffered saline (PBS) Phosphate‐buffered saline containing potassium (KPBS) PMSF (phenylmethylsulfonyl fluoride), 100 mM Potassium acetate buffer, 0.1 M Potassium phosphate buffer, 0.1 M RNase A stock solution, DNase‐free, 2 mg/ml Saline, 0.9% Saponin, 10% (w/v) SDS, 20% (w/v) SDS sample buffer SED (standard enzyme diluent) Sodium acetate, 3 M Sodium acetate buffer, 0.1 M Sodium phosphate buffer, 0.1 M Silanized glassware SSC (sodium chloride/sodium citrate), 20× SSPE (sodium chloride/sodium phosphate/EDTA), 20× T4 DNA ligase buffer, 10× TAE buffer, 50× TBE (Tris/borate/EDTA) buffer, 10× TBS (Tris‐buffered saline) TCA (trichloroacetic acid), 100% (w/v) TE (Tris/EDTA) buffer TEA (triethanolamine) solution Tris⋅Cl, 1 M Triton X‐100, 10% (w/v) TTBS (Tween 20/TBS) Urea loading buffer, 2×