Common Mistakes While Performing an Embryo Biopsy

Abstract

Embryo biopsy for Pre-Implantation Genetic Testing (PGT) is an advanced intervention in embryology lab requiring technical preciseness. Improper technique can influence the process of genetic evaluation, genetic report, and the reproductive outcomes. Following are few tips and tricks which help in optimizing the embryo biopsy technique : — It is not recommended to perform embryo biopsy in the same lab where regular embryo cultures are done. Use of UV light and Ethanol for sterilization of consumables might increase the VOCs (volatile organic compounds) and this might affect the embryo development. — Skill of micro-manipulation for an embryologist is very essential for a smooth biopsy technique — Additional care needs to be exercised when inner cell mass is at the site of biopsy or embryo has completely hatched — Negative suction pressures in the biopsy and holding pipettes during biopsy technique have to be well controlled. This avoids damage to the tissues. — Minimum of 6-8 trophectoderm cells need to be biopsied — Post biopsy we need to ensure that there is no remnant tissue on the biopsy pipette. This might bring about cross contamination of DNA. — While tubing care needs to be exercised to avoid excess buffer in the PCR tube. This can increase the chances of “NO DNA” detection. — Post biopsy its preferable to wait for one hour to assess significant damages to the embryo before vitrification — Embryos that have completely hatched need additional care at vitrification. Trophectoderm tissue is very sticky and might get adherent to the culture dish or the cryo-device. — Biopsied tissue post tubing needs to be stored at minus 20 degrees C till it gets shipped for genetic evaluation.